17:I[66739,["/_next/static/chunks/0.3dg.jzl0xwf.js?dpl=dpl_ADavNnop8X1LgnurSAwpmKs3ySqi","/_next/static/chunks/05hx6kuf6-oy4.js?dpl=dpl_ADavNnop8X1LgnurSAwpmKs3ySqi","/_next/static/chunks/0e~mn1op2jzz5.js?dpl=dpl_ADavNnop8X1LgnurSAwpmKs3ySqi","/_next/static/chunks/0juapqyy99ga..js?dpl=dpl_ADavNnop8X1LgnurSAwpmKs3ySqi","/_next/static/chunks/09-r7sdqv1jf4.js?dpl=dpl_ADavNnop8X1LgnurSAwpmKs3ySqi","/_next/static/chunks/06dn~uer6.43l.js?dpl=dpl_ADavNnop8X1LgnurSAwpmKs3ySqi"],"default"] 19:I[38632,["/_next/static/chunks/0qqtp3r0h7kah.js?dpl=dpl_ADavNnop8X1LgnurSAwpmKs3ySqi","/_next/static/chunks/0iukvycbd42rl.js?dpl=dpl_ADavNnop8X1LgnurSAwpmKs3ySqi"],"IconMark"] 18:T521,

This episode of MCAT Basics covers several separation and purification techniques you may see on the MCAT. We’ll cover these techniques in three distinct categories: small molecules, proteins, and cells.

Within the small molecule category, we’ll discuss extraction, crystallization and recrystallization, and chromatography (TLC and HPLC).

For proteins, we cover SDS-page, precipitation, soelectric focusing, and chromatography variations. Lastly, we’ll explain, within the world of cells, centrifugation, flow cytometry, and microfluidics.

Visit MedSchoolCoach.com for more help with the MCAT.

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[00:00] MCAT Tutorig from MedSchoolCoach

[00:34] Welcome to MCAT Basics

[01:07] Topics covered in this episode

[02:24] Techniques for small molecules - extraction, crystallization and recrystallization, and chromatography (TLC and HPLC)

[28:24] Techniques for proteins - SDS-page, precipitation, isoelectric focusing, and chromatography variations

[54:47] Techniques for cells - centrifugation, flow cytometry, and microfluidics.

Separation and Purification Techniques

MCAT Basics (from MedSchoolCoach)

Separation and Purification Techniques